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Designer: xiiaOmiie |
Practical manual
Hey guys!For those who have printed your practical manual earlier, before Amir's email, just wanna tell you guys, that the only changes was in Practical 6: Protein Determination (Bradford Analysis). i know it might be redundant as you guys can just reprinted it, but for those who doesn't wanna waste $$, here you go. . . Add these: - Materials > Fresh homogenised whole milk > Whey Protein concentrate powder (WPC80 Fonterra) - Procedure > 13. When reading absorbance: a) zero the spectrophotometer with standard curve blank. b) read absorbance of all samples at 595nm. c) read the standard curve tubes from low to high & then read your unknown samples. d) ensure that the outside of he curve is free of moisture and smudges prior to inserting it in the spectrophotometer for reading. > 14. Plot your standard curve and estimate the protein of your samples. Compare the measured results with those of actual values. -Questions > 2. What is the best way to prevent melamine contributing to the measured protein value? remember: smile alwaez -nabilah |